Publications

1995, The Discovery Of HAMLET's Effect-PNAS 1995-92-80642000, Antibacterial Effects-A Folding Variant Of α-lactalbumin With Bactericidal Activity Against Streptococcus Pneumoniae2000, The Definition Of The HAMLET Complex-PNAS 2000-97-42212002, Defining Oleic Acid As The Lipid Cofactor-Svensson Lipid 20022003, HAMLET Targets Nuclear Histones-duringer2004, Brain Tumor Therapy-Fischer And Gustafsson2004, Brain Tumor Therapy-Fischer And Gustafsson Et Al2004, Clinical Study Of Human Skin Papillomas-Gustafsson 20042005, Review, Advances Published2005, Review-Advances Final 52006, Cell Death Is Not By Apoptosis-HALLGREN 0410282006, Cell Death Is Not By Apoptosis-HALLGREN 041028 Published2006, Review-HAMLET, Protein Folding, And Tumor Cell Death2006, Species Variation Of Alpha-lactalbuminsPettersson, BBRC2007, Histone Acetylation-HDAC-acc2007, Histone Acetylation-HDAC-acc Published2007, Study Of Human Bladder Cancer- Mossberg2008, Review-Annals Of Medicine Pettersson-Kastberg 20082009, Treatment Of Bladder Cancer-Mossberg Et Al Bladder2010, Lipid Membranes And Perturbs Their Structure And Integrity2010, Review, Structure - Mossberg Et Al2011, Håkansson Et Al2011, Oncogene Dependence And Metabolic Paralysis-Oncogene2012, HAMLET Structure-Plos One,2812122012, Review-HAMLET-functional Properties And Therapeutic Potential2013, A Unifying Mechanism For Cancer Cell Death Through Ion Channel Activation By HAMLET2013, Colon Cancer Highlight2013, Colon Cancer Treatment-Gut-2013-Puthia-gutjnl-2012-3037152015, Kinase Inhibition—a Conserved Tumor Cell Death Mechanism Oncogene2015, The Molecular Motor F-ATP Synthase Is Targeted By The Tumoricidal Protein HAMLETProtein Receptor-independent Plasma Membrane Remodeling By HAMLET- A Tumoricidal Protein-lipid ComplexProtein-dependent Membrane Interaction of A Partially Disordered Protein Complex with Oleic Acid: Implications for Cancer LipidomicsTargeting of nucleotide-binding proteins by HAMLET—a conserved tumor cell death mechanism

P1.       Hakansson, A., Zhivotovsky, B., Orrenius, S., Sabharwal, H., Svanborg, C. 1995. Apoptosis induced by a human milk protein. Proc Natl Acad Sci U S A 92:8064.

P2.       Hakansson, A., J. Andreasson, B. Zhivotovsky, D. Karpman, S. Orrenius, C. Svanborg. 1999. Multimeric alpha-lactalbumin from human milk induces apoptosis through a direct effect on cell nuclei. Exp Cell Res 246:451.

P3.       Svensson, M., H. Sabharwal, A. Hakansson, A. K. Mossberg, P. Lipniunas, H. Leffler, C. Svanborg, and S. Linse. 1999. Molecular characterization of alpha-lactalbumin folding variants that induce apoptosis in tumor cells. J Biol Chem 274:6388.

P4.       Kohler, C., A. Hakansson, C. Svanborg, S. Orrenius, and B. Zhivotovsky. 1999. Protease activation in apoptosis induced by MAL. Exp Cell Res 249:260.

P5.       Hakansson, A., M. Svensson, A. K. Mossberg, H. Sabharwal, S. Linse, I. Lazou, B. Lonnerdal, and C. Svanborg. 2000. A folding variant of alpha-lactalbumin with bactericidal activity against Streptococcus pneumoniae. Mol Microbiol 35:589.

P6.       Svensson, M., A. Hakansson, A. K. Mossberg, S. Linse, and C. Svanborg. 2000. Conversion of alpha-lactalbumin to a protein inducing apoptosis. Proc Natl Acad Sci U S A 97:4221.

P7.       Kohler, C., V. Gogvadze, A. Hakansson, C. Svanborg, S. Orrenius, and B. Zhivotovsky. 2001. A folding variant of human alpha-lactalbumin induces mitochondrial permeability transition in isolated mitochondria. Eur J Biochem 268:186.

P8.       Svensson, M., A. K. Mossberg, J. Pettersson, S. Linse, and C. Svanborg. 2003. Lipids as cofactors in protein folding: stereo-specific lipid-protein interactions are required to form HAMLET (human alpha-lactalbumin made lethal to tumor cells). Protein Sci 12:2805.

P9.       Svensson, M., J. Fast, A. K. Mossberg, C. Duringer, L. Gustafsson, O. Hallgren, C. L. Brooks, L. Berliner, S. Linse, and C. Svanborg. 2003. Alpha-lactalbumin unfolding is not sufficient to cause apoptosis, but is required for the conversion to HAMLET (human alpha-lactalbumin made lethal to tumor cells). Protein Sci 12:2794.

P10.     Duringer, C., A. Hamiche, L. Gustafsson, H. Kimura, and C. Svanborg. 2003. HAMLET Interacts with Histones and Chromatin in Tumor Cell Nuclei. J Biol Chem 278:42131.

P11.     Fischer, W., L. Gustafsson, A. K. Mossberg, J. Gronli, S. Mork, R. Bjerkvig, and C. Svanborg. 2004. Human alpha-lactalbumin made lethal to tumor cells (HAMLET) kills human glioblastoma cells in brain xenografts by an apoptosis-like mechanism and prolongs survival. Cancer Res 64:2105.

P12.     Casbarra, A., L. Birolo, G. Infusini, F. Dal Piaz, M. Svensson, P. Pucci, C. Svanborg, and G. Marino. 2004. Conformational analysis of HAMLET, the folding variant of human alpha-lactalbumin associated with apoptosis. Protein Sci 13:1322.

P13.     Baltzer, A., C. Svanborg, and R. Jaggi. 2004. Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of alpha-lactalbumin. Cell Mol Life Sci 61:1221.

P14.     Gustafsson, L., I. Leijonhufvud, A. Aronsson, A. K. Mossberg, and C. Svanborg. 2004. Treatment of skin papillomas with topical alpha-lactalbumin-oleic acid. N Engl J Med 350:2663.

P15.     Fast, J., Mossberg, A. K., Svanborg, C., Linse, S. 2005. Stability of HAMLET–a kinetically trapped alpha-lactalbumin oleic acid complex. Protein Sci 14:329.

P16.     Fast, J., Mossberg, A. K., Nilsson, H., Svanborg, C., Akke, M., Linse, S. 2005. Compact oleic acid in HAMLET. FEBS Lett 579:6095.

P17.     Hallgren, O., Gustafsson, L., Irjala, H., Selivanova, G., Orrenius, S., Svanborg, C. 2006. HAMLET triggers apoptosis but tumor cell death is independent of caspases, Bcl-2 and p53. Apoptosis 11:221.

P18.     Pettersson, J., Mossberg, A.-K., Svanborg. C. 2006. alpha-Lactalbumin species variation, HAMLET formation, and tumor cell death. Biochem Biophys Res Commun 345:260.

P19.     Mossberg, A. K., Wullt, B., Gustafsson, L., Mansson, W., Ljunggren, E., Svanborg, C. 2007. Bladder cancers respond to intravesical instillation of HAMLET (human alpha-lactalbumin made lethal to tumor cells). Int J Cancer 121:1352.

P20.     Brest, P., Gustafsson, M., Mossberg, A. K., Gustafsson, L., Duringer, C., Hamiche, A., Svanborg, C. 2007. Histone deacetylase inhibitors promote the tumoricidal effect of HAMLET. Cancer Res 67:11327.

 P21.     Aits, S., Gustafsson, L., Hallgren, O., Brest, P., Gustafsson, M., Trulsson, M., Mossberg, A. K., Simon, H.-U., Mograbi, B. and Svanborg, C. 2009. HAMLET (human alpha-lactalbumin made lethal to tumor cells) triggers autophagic tumor cell death. Int J Cancer 124:1008.

P22.     Gustafsson, L., Aits, S., Onnerfjord, P., Trulsson, M., Storm, P., Svanborg, C. 2009. Changes in proteasome structure and function caused by HAMLET in tumor cells. PLoS ONE 4:e5229.

P23.     Pettersson-Kastberg J, Mossberg AK, Trulsson M, Yong YJ, Min S, Lim Y, O’Brien JE, Svanborg C, Mok KH. 2009. Alpha-Lactalbumin, engineered to be nonnative and inactive, kills tumor cells when in complex with oleic acid: a new biological function resulting from partial unfolding. J Mol Biol. 394(5):994.

P24.     Mossberg, A.-K., Hou, Y., Svensson, M., Holmqvist, B., Svanborg, C. 2010. HAMLET treatment delays bladder cancer development. J. Urology 183:1590.

P25.     Mossberg, A.-K., Puchades, M., Halskau, O., Baumann, A., Lanekoff, I., Chao, Y., Martinez A., Svanborg, C., Karlsson, R. 2010. HAMLET interacts with lipid membranes and perturbs their structure and integrity. PLoS ONE 2:e9384.

 P26.     Trulsson, M., Yu, H., Gisselsson, L., Chao, Y., Urbano, A., Aits, S., Mossberg, AK, Svanborg, C. 2011. HAMLET binding to α-actinin facilitates tumor cell detachment. PLoS ONE 3:e17179.

 P27.     Hakansson, A., Roche-Hakansson, H., Mossberg, AK., Svanborg, C. 2011. Apoptosis-like death in bacteria induced by HAMLET, a human milk lipid-protein complex. PLoS ONE 3:e17717.

P28.     P Storm, S Aits, M K Puthia, A Urbano, T Northen, S Powers, B Bowen, Y Chao, W Reindl, D Y Lee, N L Sullivan, J Zhang, M Trulsson, H Yang, J D Watson and C Svanborg. 2011. Conserved features of cancer cells define their sensitivity of HAMLET-induced death; c-Myc and glycolysis. Oncogene, 30, 4765–4779

P29.     Baumann A, Gjerde AU, Ying M, Svanborg C, Holmsen H, Glomm WR, Martinez A, Halskau O. 2012. HAMLET forms annular oligomers when depostited with phospholipid monolayers. J Mol Biol 418(1-2): 90-102.

P30.     Ho CS J, Rydstrom A, Manimekalai MSS, Svanborg C, Grüber G. 2012. Low resolution solution structure of HAMLET and the importance of its alpha-domains in tumoricidal activity. PLoS ONE 7(12): e53051

P31.     Puthia M, Storm P, Nadeem A, Hsiung S, Svanborg C. 2014. Prevention and treatment of colon cancer by peroal administration of HAMLET (human α-lactalbumin made lethal to tumour cells). Gut, 63: 131-42.

P32.     Smith K. 2013. Therapy: HAMLET takes a leading role on the colorectal cancer stage. Nat. Rev. Gastroenterol. Hepatol. 10:126

P33.     Xie Y, Min S, Harte NP, Kirk H, O’Brien JE, Voorheis HP, Svanborg C, Hun Mok K. 2013. Electrostatic interactions play an essential role in the binding of oleic acid with α-lactalbumin in the HAMLET-like complex: A study using charge-specific chemical modifications. Proteins. 81(1): 1-17

P34.     Storm P, Kjaer Klausen T, Trulsson M, Ho CS J, Dosnon M, Westergren T, Chao YX, Rydstrom A, Yang H, Pedersen SF, Svanborg C .2013. A Unifying Mechanism for Cancer Cell Death through Ion Channel Activation by HAMLET. PLoS ONE 8(3): e58578.

P35.     James Ho CS, Petter Storm, Anna Rydstrom, Ben Bowen, Fredrik Alsin, Louise Sullivan, Ines Ambite, Ken Hun Mok, Trent Northen and Catharina Svanborg. 2013. Lipids as Tumoricidal Components of Human Alpha-lactalbumin Made Lethal to Tumor Cells (HAMLET); Unique and Shared Effects on Signaling and Death. J Biol Chem. doi:10.1074/jbc.M113.468405

P36.     Ho James, Sielaff H, Nadeem A, Svanborg C, Gruber G. 2015. The molecular motor F-ATP synthase is targeted by the tumoricidal protein HAMLET. J Mol Biol. 427(10):1866-74. doi: 10.1016/j.jmb.2015.01.024.

P37.     Ho, J., Nadeem, A., Rydström, A., Puthia, M and Svanborg, C. 2015. Targeting of nucleotide-binding proteins by HAMLET – a conserved tumor cell death mechanism. Oncogene. doi: 10.1038/onc.2015.144.

SM1.    Aftab Nadeem, James Ho C.S., Jeremy Sanborn, Anna Rydström, Douglas L. Gettel, Viviane N. Ngassam, Thomas Kjær Klausen, Stine Falsig Pedersen, Atul N. Parikh and Catharina Svanborg. Receptor-independent plasma membrane remodeling by HAMLET; a tumoricidal protein-lipid complex. Submitted.

SM2.    James Ho CS, Jackwee Lim, Anna Ryström, Catharina Svanborg and Gerhard Grüber. Distinct peptide-oleate structures of the tumoricidal HAMLET complex alter ion-flux and gene expression in tumor cells. Submitted.

R1.       Svensson, M., C. Duringer, O. Hallgren, A. K. Mossberg, A. Hakansson, S. Linse, and C. Svanborg. (2002) Hamlet–a complex from human milk that induces apoptosis in tumor cells but spares healthy cells. Adv Exp Med Biol 503:125.

R2.       Svanborg, C., H. Agerstam, A. Aronson, R. Bjerkvig, C. Duringer, W. Fischer, L. Gustafsson, O. Hallgren, I. Leijonhuvud, S. Linse, A. K. Mossberg, H. Nilsson, J. Pettersson, and M. Svensson. 2003. HAMLET kills tumor cells by an apoptosis-like mechanism–cellular, molecular, and therapeutic aspects. Adv Cancer Res 88:1.

R3.       Duringer, C., Hallgren, O., Gustafsson, L., Pettersson, J., Mossberg, A. K., Manolov, T. and Svanborg, C. 2005. HAMLET; a novel tool to identify apoptotic pathways in tumor cells. in Sluyser, M. (Ed), Application of apoptosis to cancer treatment., Springer, pp. 223-246.

R4.       Gustafsson, L., Hallgren, O., Mossberg, A. K., Pettersson, J., Fischer, W., Aronsson, A. and Svanborg, C. 2005. HAMLET Kills Tumor Cells by Apoptosis: Structure, Cellular Mechanisms, and Therapy. J Nutr 135:1299.

R5.       Mok, K. H., Pettersson, J., Orrenius, S. and Svanborg, C. 2007. HAMLET, protein folding, and tumor cell death. Biochem Biophys Res Commun 354:1.

 R6.       Hallgren, O., Aits, S., Brest, P., Gustafsson, L., Mossberg, A. K., Wullt, B. and Svanborg, C. 2008. Apoptosis and tumor cell death in response to HAMLET (human alpha-lactalbumin made lethal to tumor cells). Adv Exp Med Biol 606:217.

R7.       Pettersson-Kastberg, J., Aits, S., Gustafsson L., Mossberg A., Storm, P., Trulsson, M., Persson, F., Mok, K. H. and Svanborg, C. 2009. Can misfolded proteins be beneficial? The HAMLET case. Ann med 41:162.

R8.       Svanborg, C., Aits, S., Brest, P., Gustafsson, L., Mok, K., Moreillhon, C., Mossberg, A., Pettersson, J., Storm, P. and Trulsson, M. 2009. HAMLET, a tumoricidal molecular complex from human milk. BMC Proceedings 3(Suppl 5):S11

R9.       Mossberg, A. K., Mok, K. H., Morozova-Roche, L. A. and Svanborg, C. 2010. Structure and function of human a-lactalbumin made lethal to tumor cell (HAMLET)-type complexes. FEBS Journal 277:4614.

P10.     Min S, Meehan J, Sullivan LM, Harte NP, Xie Y, Davey GP, Svanborg C, Brodkorb A, Mok KH. (2012) Alternatively folded proteins with unexpected beneficial functions. Biochem Soc Trans. 40(4):746-751.

P11.     Ho CS J, Rydström A, Trulsson M, Bålfors J, Storm P, Puthia M, Nadeem A and Svanborg C. 2012. HAMLET: functional properties and therapeutic potential. Future Oncol. 8(10), 1301-1313